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KMID : 0857020100250010010
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Kosin Medical Journal
2010 Volume.25 No. 1 p.10 ~ p.19
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Expression Patternof Antigen Presenting Cells and GFAP in Rat Brain after Cryoinjury
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Kang Mi-Young
Kim Kang-Ryune
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Abstract
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Background : Traumatic brain injury is a multifaceted injury that involves direct mechanical damage, intraparenchymal
and subarachnoid hemorrhage, breakdown of the blood-brain barrier, excitotoxicity, and ischemia. Even though much
investigations were performed, acceptable mechanical informations were rare. The aim of this study was to reveal the
migration of antigen presenting cells associated with inflammation and the expression pattern of GFAP associated with
gliotic scars in cerebral cortex of rats after cryoinjury.
Material & Method : A total of 18 male Sprague-Dawley rats weighing 300 g, 2 months old, were used throughout
the experiments. To injure the brain, rats were anesthetized for surgery with 3.5% chloral hydrate(1 ㎖/100 g,
intraperitoneally); the frontal bones were exposed by elevating the skin; and craniectomies were performed adjacent
to the central suture, midway between lambda and bregma. A cryoinjury was then created by applying a cold probe
(3-mm-diameter steel rod chilled in liquid nitrogen) to the left frontal cortex (ipsilateral cortex) for 1 minute. Rats
were sacrificed at 6 hours, 1, 4, 7 and 14 days postsurgery (n=3, per time point), and three rats were sacrificed as
normal controls. Serial brain cryosections were made by cryostat. For immunohistochemistry, brain tissue sections were
allowed to react with mouse anti-rat MHC class II antibody(1:500), mouse anti-rat ED2 antibody(1:500), and mouse
anti-rat GFAP antibody(1:200).
Results : MHC class II positive dendritic cells were absent in normal cerebral cortex parenchyme, but were more 16
times number in injured rats at 7 days after cryoinjury. ED2 positive macrophages were rare in normal cerebral cortex
parenchyme, and were more 14 times number in injured rats at 4 days after cryoinjury. GFAP positive astrocytes were
not observed in normal rats, and increased suddenly at 4 days rats after cryoinjury. GFAP positive neurons were not
expressed in normal rats, and in cryoinjured rats.
Conclusion : In conclusion, the above results suggest that the increases of the number of the two kinds of the
antigen-presenting cells affect cell-mediated immune responses and phagocytosis, and GFAP positive cells attends in the
formation of gliotic scars.
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KEYWORD
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Antigen presenting cell, Dendritic cell, Macrophage, GFAP, Cryoinjury, Rat brain
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